Fig 1: MFN2 affects NF-?B signaling through cytosolic expression of IRAK4 signaling complex. (A) Western blot analysis and quantification of TAK1 phosphorylation in control and MFN2-silenced macrophages stimulated with zymosan (50 µg/ml) for 30 min. (B) Western blot analysis and quantification of IRAK4 phosphorylation in control and MFN2-silenced macrophages stimulated with zymosan (50 µg/ml) for 30 min. (C) Western blot analysis and quantification of IRAK4 and IRAK1 in control and MFN2-silenced macrophages stimulated with zymosan (50 µg/ml) for 30 min. (D) mRNA expression of IRAK4 and IRAK1 in control and MFN2-silenced macrophages. (E–G) Western blot analysis and quantification of TRAF6 (E), IRAK1 (F), and IRAK4 (G) proteins in cytosolic and mitochondrial fractions from zymosan-treated control and MFN2-silenced macrophages. All results are shown as means ± SEM of at least three independent experiments compared using one-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test or Student unpaired, two-tailed t-test; *p < 0.05; **p < 0.01; ns, not significant.